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2 edition of Initial characterization of the Escherichia coli K-12 metjtsl gene product and its effect(s) on the metF transcription unit found in the catalog.

Initial characterization of the Escherichia coli K-12 metjtsl gene product and its effect(s) on the metF transcription unit

Gregory A Bala

Initial characterization of the Escherichia coli K-12 metjtsl gene product and its effect(s) on the metF transcription unit

by Gregory A Bala

  • 336 Want to read
  • 7 Currently reading

Published .
Written in English

    Subjects:
  • Escherichia coli -- Genetics,
  • Genetic transcription

  • Edition Notes

    Statementby Gregory A. Bala
    The Physical Object
    Paginationvii, 90 leaves :
    Number of Pages90
    ID Numbers
    Open LibraryOL14421146M

    Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /), also known as E. coli (/ ˌ iː ˈ k oʊ l aɪ /), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in Class: Gammaproteobacteria. › cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli Strains i › K12,K › K12 / A19 › K12 / AB,AB › K12 / ACC5 › K12 / AN More».

      Transcriptional regulatory networks allow bacteria to express proteins only when they are needed. Adaptive hypotheses explaining the evolution of regulatory networks assume that unneeded expression is costly and therefore decreases fitness, but the proximate cause of this cost is not clear. We show that the cost in fitness to Escherichia coli strains constitutively expressing the lactose Cited by: gene yggS: polypeptide PLP homeostasis protein. Escherichia coli K substr. MG Accession IDs: G (EcoCyc) b ECK P (UniProt) Length: bp / aa: Map Position [3,, -> 3,,] ( centisomes, °) Location: cytosol Report Errors .

    Find + million publication pages, 20+ million researchers, and k+ projects. onAcademic is where you discover scientific knowledge and share your research. Escherichia coli is a predominant species among facultative anaerobic bacteria of the gastrointestinal tract. Both its frequent community lifestyle and the availability of a wide array of genetic tools contributed to establish E. coli as a relevant model organism for the study of surface colonization. Several key factors, including different extracellular appendages, are implicated in E. coli Cited by:


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Initial characterization of the Escherichia coli K-12 metjtsl gene product and its effect(s) on the metF transcription unit by Gregory A Bala Download PDF EPUB FB2

INTRODUCTION. Escherichia coli strain K is arguably the single organism about which the most is known. Originally isolated init was catapulted to prominence by the discovery of strain K's ability to carry out genetic recombination by conjugation and, soon after, by generalized transduction ().The strain K has been widely distributed to laboratories across the by: The water-accessible volumes, the amounts of all significant osmolytes, and the protein concentration in the cytoplasm of aerobically grown Escherichia coli K have been determined as a function of the osmolarity of the minimal growth medium.

The volume of cytoplasmic water (V ̄ cyto) decreases linearly with increasing osmolarity from (±) μl/mg dry weight in cells grown at Cited by: Abstract. A morphological mutant of Escherichia coli K that grows as round cells at 30, 37, or 42 C in a variety of complex and synthetic media has been isolated and characterized.

The gene concerned, designated rodA, has been shown to be on the chromosome between the purE and pyrC loci and to be located at about minute The rodA gene has been found to be co-transducible with the lip Cited by: Identification and Characterization of a New Gene of Escherichia coli K Involved in Outer Membrane Permeability Barbara A.

Sampson,' Rajeev Misra and Spencer A. Benson' Department of Biology, Princeton University, Princeton, New Jersey 4 Manuscript received Novem Accepted for publication Ma ABSTRACTCited by: The excC mutants of Escherichia coli are hypersensitive to drugs such as cholic acid and release periplasmic proteins Into the extracellular medium.

A bp fragment carrying the excC gene was isolated and sequenced. It contains the 3′ end of the tolB gene which maps at min 17 on the E.

coll map and an open reading frame which encodes the Da ExcC by: Participation of Escherichia coli K groE gene products in the synthesis of cellular DNA and RNA As it is described in this book chapter, while N2 fixation has been well characterized under.

The csrA gene may affect succinate utilization or transport, independently of its effect on gluconeogenesis. The csrA gene is located at 58 min on the physical map of the E. coli K genome. It appears between the gene alaS, which encodes alanyl tRNA synthetase (EC ), and the serV operon of tRNA genes, and is transcribed.

coli K is a rod-shaped bacterium measuring a few microns in length and µm wide. Being a prokaryote, it lacks a nuclear membrane. Its genes reside on a single circular, double-stranded DNA molecule tightly packed within the cytosol of the cell (Fig.

1).However, it should be noted that pathogenic E. coli strains such as OH7 have many more genes (). The yiaMNO genes of Escherichia coli K encode one family member that transports the rare pentose l-xylulose.

Its physiological role is unknown, since wild-type E. coli K does not utilize l. 14 Plasmid Regulation and Systems-Level Effects on Escheric hia coli Metabolism Liu F, Huang L () De velopment of non-viral vectors for systemic gene delivery.

J Control. Bacterial strains and growth conditions. The E. coli K strains that were used in this study are listed in Table Table1. growth of these strains as unchallenged, challenged nonadapted, and challenged preadapted cultures, a single colony was inoculated into 5 ml Luria broth (LB) and grown with aeration at 37°C for 8 to 10 h (to an A of at least ).Cited by: Our genetic analysis showed that katF carries an amber mutation in E.

coli K and many of its derivatives but that katF has reverted to an active form in the much-used strain MC   The 4,–base pair sequence of Escherichia coli K is presented. Of protein-coding genes annotated, 38 percent have no attributed function. Comparison with five other sequenced microbes reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E.

coli are also evident. The largest family of paralogous proteins contains 80 Cited by: Characterization of Escherichia coli bacterial viruses in commercial sera lambda-infected Escherichia coli K Appl.

Microbiol. 16 T4 gene 16 and 17 products appear to be necessary for. INTRODUCTION. Escherichia coli K is clearly one of the best studied organisms and has had an enormous contribution on construction of concept of genes over the past half century ().It is, however, still far from completely understood at the systems level, although complete genome structure was established and numerous functional analyses have been by:   The Tol-Pal proteins of Escherichia coli are involved in maintaining outer membrane integrity.

They form two complexes in the cell envelope. Transmembrane domains of TolQ, TolR, and TolA interact in the cytoplasmic membrane, while TolB and Pal form a complex near the outer membrane. The N-terminal transmembrane domain of TolA anchors the protein to the cytoplasmic membrane and Cited by: While using Escherichia coli for mammalian protein expressions, a new NADP +-dependent ALDH was found (Ho and Weiner ).Its properties were that of a typical class 2 enzyme.

The E. coli literature described two other ALDHs that were involved in specific metabolic pathways rather than being a general detoxifying enzyme like so many others (Caballero et al. ; Hanlon et al. ).Cited by: The Essential Genome of Escherichia coli K Emily C. Goodall, aAshley Robinson, product of the disrupted gene is required for normal rates of growth under the conditions tested.

In the current study, to minimize the possibility of incorrectly Individual colonies were pooled to construct the initial library,Cited by: The expression of the metA gene coding for the first enzyme in the methionine biosynthethic pathway was studied in wild-type and in deregulated strains of Escherichia coli K carrying the gene on multicopy plasmids.

We looked at (a) in vitro activity of the metA product—The enzyme homoserine transsuccinylase (HTS); (b) resistance of cells carrying metA plasmids to the analogue α Cited by: 6. Escherichia coli (/ ˌ ɛ ʃ ɪ ˈ r ɪ k i ə ˈ k oʊ l aɪ /; commonly abbreviated E.

coli) is a Gram-negative gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms). The descendants of two isolates, K and B strain, are used routinely in molecular biology as both a tool and a model organism.

We have systematically made a set of precisely defined, single‐gene deletions of all nonessential genes in Escherichia coli K‐ Open‐reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one‐step method for inactivation of chromosomal genes and primers designed to create in‐frame deletions upon excision of the Cited by: Escherichia coli K–12 and B strains are among the most frequently used bacterial hosts for production of recombinant proteins on an industrial scale.

To improve existing processes and to accelerate bioprocess development, we performed a detailed host analysis. We investigated the different behaviors of the E. coli production strains BL21, RV, and HMS in response to high-glucose Cited by: About the same activity was measured against Escherichia coli K, which reversibly changes its morphology to mostly elongated forms along the major axis.

Perhaps, there is a relation to the MreB activity of another pyrrole–imidazole alkaloid, (−)-sceptrin ((−)- 19).